Paper microzone plates.
نویسندگان
چکیده
This paper describes 96- and 384-microzone plates fabricated in paper as alternatives to conventional multiwell plates fabricated in molded polymers. Paper-based plates are functionally related to plastic well plates, but they offer new capabilities. For example, paper-microzone plates are thin (approximately 180 microm), require small volumes of sample (5 microL per zone), and can be manufactured from inexpensive materials ($0.05 per plate). The paper-based plates are fabricated by patterning sheets of paper, using photolithography, into hydrophilic zones surrounded by hydrophobic polymeric barriers. This photolithography used an inexpensive formulation photoresist that allows rapid (approximately 15 min) prototyping of paper-based plates. These plates are compatible with conventional microplate readers for quantitative absorbance and fluorescence measurements. The limit of detection per zone loaded for fluorescence was 125 fmol for fluorescein isothiocyanate-labeled bovine serum albumin, and this level corresponds to 0.02 the quantity of analyte per well used to achieve comparable signal-to-noise in a 96-well plastic plate (using a solution of 25 nM labeled protein). The limits of detection for absorbance on paper was approximately 50 pmol per zone for both Coomassie Brilliant Blue and Amaranth dyes; these values were 0.4 that required for the plastic plate. Demonstration of quantitative colorimetric correlations using a scanner or camera to image the zones and to measure the intensity of color, makes it possible to conduct assays without a microplate reader.
منابع مشابه
Paper-based ELISA.
This paper describes enzyme-linked immunosorbent assays (ELISA) performed in a 96-microzone plate fabricated in paper (paper-based ELISA, or P-ELISA). ELISA is widely used in biochemical analyses; these assays are typically carried out in microtiter plates or small vials. ELISA combines the specificity of antibodies with high-turnover catalysis by enzymes to provide specificity and sensitivity....
متن کاملHigh reuse efficiency of radio resources in urban microcellular systems
A new microcell configuration scheme in urban areas is proposed in order to increase the reuse efficiency of radio resources. Multiple directional-beam antennas lined up along a street form a cigar-shaped cell. Antennas are located at intersection and are directed along the intersecting streets. Two antennas facing each other form a microzone and, therefore, a cigar-shaped cell consists of mult...
متن کاملA Novel Architecture for Antenna Arrangement in Wireless Cellular CDMA Systems
Wise arrangement of antennas is critical in wireless cellular systems for both reduction of co-channel interference (CCI) and increase the quality of service (QoS). In this paper, a novel architecture for antenna arrangement in CDMA wireless cellular systems is presented. In this architecture that we called microzone, every cell is divided into three (or more) zones and information transmission...
متن کاملOxygen dynamics in the rhizosphere of Cymodocea rotundata
The spatial distribution of oxygen and the dynamics of the oxic mlcrozone around roots of Cymodocea rotundata were studied using oxygen microelectrodes under constant light conditions and during light-dark transitions. Under daylight steady state conditions, oxygen was present at concentrations up to 75 % of air saturation at the root surface, and the oxic microzone around the roots was 80 pm t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical chemistry
دوره 81 15 شماره
صفحات -
تاریخ انتشار 2009